rabbit anti-map-2 polyclonal primary antibody  (Thermo Fisher)

Journal: BioMed Research International

Article Title: Bone Morphogenetic Protein-7 (BMP-7) Promotes Neuronal Differentiation of Bone Marrow Mesenchymal Stem Cells (BMSCs) In Vitro

doi: 10.1155/2021/7239783

Figure Lengend Snippet: Cell immunofluorescence detection. The expression and location of MAP-2 and Nfh were detected with the cell immunofluorescence (200x).

Article Snippet: After being treated with 0.5% Triton X-100 at room temperature for 20 min and the following washing, the cells were blocked with 5% BSA solution at room temperature for 20 min. Then, the cells were incubated with the rabbit anti-Nfh primary antibody (1 : 1500 dilution; bs-10680R; Bioss), or rabbit anti-MAP-2 primary antibody (1 : 1500 dilution; bs-1369R; Bioss), at 4°C overnight.

Techniques: Immunofluorescence, Expressing

Journal: BioMed Research International

Article Title: Bone Morphogenetic Protein-7 (BMP-7) Promotes Neuronal Differentiation of Bone Marrow Mesenchymal Stem Cells (BMSCs) In Vitro

doi: 10.1155/2021/7239783

Figure Lengend Snippet: Relative mRNA expression levels of MAP-2 and Nfh. (a, b) Quantitative real-time PCR was performed to detect the mRNA expression levels of MAP-2 (a) and Nfh (b). Compared with the control and lentiviral vector control groups, ∗ P < 0.05.

Article Snippet: After being treated with 0.5% Triton X-100 at room temperature for 20 min and the following washing, the cells were blocked with 5% BSA solution at room temperature for 20 min. Then, the cells were incubated with the rabbit anti-Nfh primary antibody (1 : 1500 dilution; bs-10680R; Bioss), or rabbit anti-MAP-2 primary antibody (1 : 1500 dilution; bs-1369R; Bioss), at 4°C overnight.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Plasmid Preparation

Journal: BioMed Research International

Article Title: Bone Morphogenetic Protein-7 (BMP-7) Promotes Neuronal Differentiation of Bone Marrow Mesenchymal Stem Cells (BMSCs) In Vitro

doi: 10.1155/2021/7239783

Figure Lengend Snippet: Relative protein expression levels of MAP-2 and Nfh. (a) Western blot analysis was performed to detect the protein expression levels of MAP-2 and Nfh. (b) Statistical analysis. Compared with the control and lentiviral vector control groups, ∗ P < 0.05.

Article Snippet: After being treated with 0.5% Triton X-100 at room temperature for 20 min and the following washing, the cells were blocked with 5% BSA solution at room temperature for 20 min. Then, the cells were incubated with the rabbit anti-Nfh primary antibody (1 : 1500 dilution; bs-10680R; Bioss), or rabbit anti-MAP-2 primary antibody (1 : 1500 dilution; bs-1369R; Bioss), at 4°C overnight.

Techniques: Expressing, Western Blot, Plasmid Preparation

Journal: Cells

Article Title: Vertically-Aligned Functionalized Silicon Micropillars for 3D Culture of Human Pluripotent Stem Cell-Derived Cortical Progenitors

doi: 10.3390/cells9010088

Figure Lengend Snippet: hCPs seeded on silicon micropillar arrays preserve their cortical regional identity and upon exposure to differentiative conditions, generate cortical glutamatergic neurons. ( A ) eGFP +ve hCPs cultured for 14 days on 3D silicon devices preserve the expression of the cortical progenitor marker TBR2 (red). Nuclei are stained with Hoechst (blue). Scale bar: 50 μm. ( B ) hCPs cultured for 5 days on 3D silicon devices and then exposed for 35 days to differentiative conditions maturate into cortical glutamatergic neurons. Left: cultures stained for the pan-neuronal neuronal marker β3-TUBULIN (red). Nuclei are stained with Hoechst (blue). Scale bar: 10 μm. Right: cultures stained for the mature neuronal marker MAP2 (red) and for the cortical neuronal marker CUX1 (green). Nuclei are stained with Hoechst (blue). Scale bar: 10 μm.

Article Snippet: Antibodies used in this study: primary mouse monoclonal anti-NESTIN antibody (R&D Systems, Minneapolis, MN, USA, 1:300), primary mouse monoclonal β3-TUBULIN antibody (Promega, Milan, Italy, 1:1000), primary rabbit polyclonal anti-SOX2 antibody (Millipore, Milan, Italy, 1:200), primary rabbit polyclonal anti-MAP2 antibody (Santa Cruz, Heidelberg, Germany, 1:200), primary mouse monoclonal anti-TBR2 antibody (ABCAM, Cambridge, UK, 1:500), primary mouse monoclonal anti CUX1 (ABCAM, 1:200), AlexaFluor-488 or -568 conjugated secondary antibodies (Thermo Fisher Scientific, 1:500).

Techniques: Cell Culture, Expressing, Marker, Staining